Regulatory

Part:BBa_M11544:Design

Designed by: Alyssa Calder   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-21)


Fe (iron) Promoter (point mutations)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 814
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 47
    Illegal NgoMIV site found at 397
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 421


Design Notes

There were several PstI restriction sites that had to be mutated. The six nucleotide sequence for PstI is ctgcag. We mutated it so that the sequence became ctacag, keeping the appropriate amino acid still in place, but getting rid of the potential for PstI to cut it.


Source

Part BBa_I765000

References